Lactobacilli MRS Broth is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans. Lactobacilli MRS broth was originally developed in 1960 by de Man, Rogosa & Sharpe, the medium can be used for confirmatory tests on organisms isolated on MRS Agar. The medium can also be used for enumeration by the Miles and Misra technique.

Nutrition is provided by a mixture of carefully selected peptones, glucose, beef and yeast extracts while Tween® 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci and moulds is provided by ammonium citrate and sodium acetate. Occasionally, sterilisation of this medium at 121°C for 15 minutes, in some autoclaves, may cause the pH to fall outside of the specified pH limits 6.4 ±0.2. In these rare cases, adjustment of the medium using acetic acid or sodium hydroxide is recommended.

• Analyte: Lactobacillus spp.
• Platform: dehydrated culture media
• Formula: peptone 10.0g/L, yeast extract 5.0g/L, beef extract 10.0g/L, glucose 20.0g/L, potassium phosphate 2.0g/L, sodium acetate 5.0g/L, magnesium sulfate 0.2g/L, manganese sulfate 0.05g/L, Tween 80 1.08g/L, ammonium citrate 2.0g/L
• Grams per litre: 55.00
• Litres per 500g: 9.00